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Possible involvement of Heme Oxygenase-1 in Attenuation of Diabetes Mellitus Induced Vascular Endothelial Dysfunction in Rodents
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| Author:
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SEMWAL BC, SINGH, S, YADAV HN
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| Abstract:
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Diabetes mellitus (DM) is one of the prime health problems all over the world, which is characterized by an increase in blood glucose levels, alteration in carbohydrate, fat and protein metabolism, associated with endothelial dysfunction. It is well suggested that the prevention of endothelial dysfunction gaining attention against the progression of cardiovascular diseases. Diabetes was induced by the streptozotocin and animals were randomly divided into different groups and received hemin, daidzein and L-NAME for 2 weeks. At the end of the study, animals were sacrificed and blood sample were collected in a heaparinized test tube and thoracic aorta was isolated for the measurement of Ach and SNP induced vasoralaxtion effect, assessment of oxidative stress markers, activity of antioxidant enzymes NO production and blood glucose level.
Results: Diabetic rats showed significantly increased the blood glucose and MDA level and decreased SOD and CAT activity in aortic tissues. Treatment of hemin and daidzein either alone or in combination ameliorated the STZ induced endothelail dysfunction by decreasing the blood glucose and oxidative stress markers. In addition these agents also boost up the activity of antioxidant enzyme as well as the production of eNOS dependent NO.
Conclusions: These findings suggest that treatment of hemin and daidzein decreases the vascular complications and tissue injuries induced by diabetes by decreasing oxidative stress and restoring NO bioavailability.
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| Keyword:
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Heme oxygenase-1, hemin, daidzein, oxidativev stress
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.03.046
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| Download:
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