|
Dioscorea bulbifera l. - A Novel Source for the Production of Glutamic Acid Using Statistical Optimization Method
|
|
| Author:
|
M. REDDY, USHARANI VADDADI , PAYALA VIJAYALAKSHMI
|
| Abstract:
|
The present investigation intends to develop a realistic method of maximizing the production of L-glutamic acid using a novel substrate as a carbon source Dioscorea bulbifera L or air potato through submerging bacterial fermentation by Corynebacterium glutamicum DSM 20300T and Arthrobacter globiformis MTCC 4299. In order to evaluate the effect of critical growth elements on the production, statistical optimization technique like Central Composite Design Matrix (CCD) and Response Surface Methodology (RSM) was used. In the end the product was analyzed qualitatively by thin layer chromatography and quantitatively by standard ninhydrin method. The resultant glutamic acid product was subjected to crystallization at its isoelectric pH. The product purity was confirmed by Fourier transform infrared spectroscopy. The results from multiple linear regression analysis had found that, the linear factors like urea and mineral salt solution concentration and interaction term urea and mineral salt concentration showed significant effect (p<0.05) on the glutamic acid yield by both the microbial cultures. The fisher F-test with a very low probability value (Pmodel > F= 0.0001) show a very high significance for the regression model for production with Corynebacterium glutamicum. F-value (with a 95% confidence interval) was much larger than 4 (Pmodel > F= 0.001) and low probability value for each variable and respective interaction for the glutamic acid production with Arthrobacter globiformis was significant. The explained variance (R2 = 0.93) and (R2 = 0.82) ensured adequate fit for predicted versus actual experimental values thereby validating the forecast model for glutamic acid production by Corynebacterium glutamicum and Arthrobacter globiformis. Since glutamic acid is one of the most highly produced amino acids, the current study encourages the establishment of an economic fermentation process to meet the growing demand for glutamic acid on the global market at a reasonable price.
|
| Keyword:
|
Glutamic acid, Dioscorea bulbifera L, submerged fermentation, optimization, Central Composite Design matrix, Response Surface Methodology, Crystallization.
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2020.12.03.012
|
| Download:
|
Request For Article
|
|
|