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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence
Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Polymerase chain reaction isa precise methodto revealingand diagnosis of Hepatitis Bfor Iraqi patients

Author: MINEN AL-KAFAJY
Abstract: Hepatitis B is an epidemic in Iraq; therefore, several types of research have studied hepatitis in the south of Iraq. However, none have used PCR to investigate the genetic containing of the virus in this particular area. Therefore, this project intends to detect the presence of hepatitis B using molecular methods and determine the accuracy of the available routine serological methods. The project was performed during the period between September 2017 to January 2019. The Samples have been collected from Dhi-Qar hospital clinical lab, in the province of Dhi-Qar, Iraq. The study has examined 135 patients that have been sending by the internal medicine clinic to the lab to be tested for the possibility of having hepatitis. ELIZA technique has shown that 99 (73.3%) of the patients were tested positive for hepatitis, while 36 (26.6%) are negative. Additionally, the study detected the male sex bias nature of the disease represented by 73 men infected while only 26 women were diseased in the present study. Moreover, aging was one of the factors that reduce the defense mechanism of the disease. Interestingly, PCR test has shown that seven men and ten women patients were positive while they been considered negative by ELISA. My study suggests using the PCR technique in the clinic lab as it can detect the presence of the nucleic acid in a fast and more accurate way.
Keyword: PCR, HBV, liver, Anti-HBc.
DOI: https://doi.org/10.31838/ijpr/2020.12.02.0160
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