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Polymerase chain reaction isa precise methodto revealingand diagnosis of Hepatitis Bfor Iraqi patients
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| Author:
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MINEN AL-KAFAJY
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| Abstract:
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Hepatitis B is an epidemic in Iraq; therefore, several types of research have studied hepatitis in the south of Iraq.
However, none have used PCR to investigate the genetic containing of the virus in this particular area. Therefore,
this project intends to detect the presence of hepatitis B using molecular methods and determine the accuracy of
the available routine serological methods. The project was performed during the period between September 2017
to January 2019. The Samples have been collected from Dhi-Qar hospital clinical lab, in the province of Dhi-Qar,
Iraq. The study has examined 135 patients that have been sending by the internal medicine clinic to the lab to be
tested for the possibility of having hepatitis. ELIZA technique has shown that 99 (73.3%) of the patients were
tested positive for hepatitis, while 36 (26.6%) are negative.
Additionally, the study detected the male sex bias nature of the disease represented by 73 men infected while only
26 women were diseased in the present study. Moreover, aging was one of the factors that reduce the defense
mechanism of the disease. Interestingly, PCR test has shown that seven men and ten women patients were positive
while they been considered negative by ELISA. My study suggests using the PCR technique in the clinic lab as it can
detect the presence of the nucleic acid in a fast and more accurate way.
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| Keyword:
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PCR, HBV, liver, Anti-HBc.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.02.0160
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| Download:
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Request For Article
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