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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Phytochemical investigation,anti-proliferative and antioxidant- activities of Iraqi Capparisspinosa L. (Family Capparidaceae) against MCF-7 human Breast cancer cell line

Author: ENAS JAWAD, SAMEER HASAN A., ZAHRA A. S.NASSIR
Abstract: Objective: The aim of this study was to investigate chemical constituents and antioxidant activity of aerial parts of Capparisspinosasince no phytochemical investigation had been done previously in Iraq. Methods: aerial parts of Capparisspinosawere defatted by maceration in hexane for 24 h. The defatted plant materials were extracted using Soxhlet apparatus, the aqueous methanol 85% as a solvent extraction for 24 h, and fractionated by ethylacetate . The ethyl acetate fractions for the plant were analyzed by high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) for its phenolic acid and flavonoid contents. The antioxidant activity was done on ethyl acetate fraction by DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radical scavenging assay, also this fraction was chosen to test the anti-proliferative action of against breast cancer cell line MCF-7 and rate embryonic fibroblasts (REF) normal cell line, Results: The different chromatographic results revealed the presence of different phenolic compounds in the plant extract like luteolin, rutin, astragalin, isoquercetin, catechins, and 3,5-dicaffeoylquinic acid (3,5-DCQA) and the data showed that ethyl acetate fraction has significant anti-proliferative activity against MCF-7 (IC50 =35. 5µg/ml) and REF (IC50 =1762.9 µg/ml)and possess potent antioxidant activity when compared with reference compound ascorbic acid(Vitamin C). The antioxidant activity may relate to the existence of flavonoids which had been approved as potent antioxidant, and identified by Preliminary qualitative phytochemical analysis.
Keyword: CapparisspinosaL, Flavonoids, Phenolic acid, HPLC, HPTLC, Antioxidant, Breast cancer.
DOI: https://doi.org/10.31838/ijpr/2020.12.02.0158
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