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Phytochemical investigation,anti-proliferative and antioxidant- activities of Iraqi Capparisspinosa L. (Family Capparidaceae) against MCF-7 human Breast cancer cell line
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| Author:
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ENAS JAWAD, SAMEER HASAN A., ZAHRA A. S.NASSIR
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| Abstract:
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Objective: The aim of this study was to investigate chemical constituents and antioxidant activity of aerial parts of
Capparisspinosasince no phytochemical investigation had been done previously in Iraq.
Methods: aerial parts of Capparisspinosawere defatted by maceration in hexane for 24 h. The defatted plant
materials were extracted using Soxhlet apparatus, the aqueous methanol 85% as a solvent extraction for 24 h, and
fractionated by ethylacetate . The ethyl acetate fractions for the plant were analyzed by high-performance liquid
chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) for its phenolic acid and
flavonoid contents. The antioxidant activity was done on ethyl acetate fraction by DPPH (2, 2-diphenyl-1-picrylhydrazyl)
free
radical
scavenging
assay,
also
this
fraction
was
chosen
to
test
the
anti-proliferative
action
of
against
breast
cancer
cell
line
MCF-7
and
rate
embryonic
fibroblasts
(REF)
normal
cell
line,
Results:
The different chromatographic results revealed the presence of different phenolic compounds in the
plant extract like luteolin, rutin, astragalin, isoquercetin, catechins, and 3,5-dicaffeoylquinic acid (3,5-DCQA) and
the data showed that ethyl acetate fraction has significant anti-proliferative activity against MCF-7 (IC50 =35.
5µg/ml) and REF (IC50 =1762.9 µg/ml)and possess potent antioxidant activity when compared with reference
compound ascorbic acid(Vitamin C). The antioxidant activity may relate to the existence of flavonoids which had
been approved as potent antioxidant, and identified by Preliminary qualitative phytochemical analysis.
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| Keyword:
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CapparisspinosaL, Flavonoids, Phenolic acid, HPLC, HPTLC, Antioxidant, Breast cancer.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.02.0158
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| Download:
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Request For Article
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