*Five Years Citation in Google scholar (2016 - 2020) is. 1451*   *    IJPR IS INDEXED IN ELSEVIER EMBASE & EBSCO *       

logo

INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence
Published by : Advanced Scientific Research
ISSN
0975-2366
Current Issue
Article In Press
No Data found.
ADOBE READER

(Require Adobe Acrobat Reader to open, If you don't have Adobe Acrobat Reader)

Index Page 1
Click here to Download
IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

Click to download
 

Article Detail

Label
Label
Phytochemical investigation,anti-proliferative and antioxidant- activities of Iraqi Capparisspinosa L. (Family Capparidaceae) against MCF-7 human Breast cancer cell line

Author: ENAS JAWAD, SAMEER HASAN A., ZAHRA A. S.NASSIR
Abstract: Objective: The aim of this study was to investigate chemical constituents and antioxidant activity of aerial parts of Capparisspinosasince no phytochemical investigation had been done previously in Iraq. Methods: aerial parts of Capparisspinosawere defatted by maceration in hexane for 24 h. The defatted plant materials were extracted using Soxhlet apparatus, the aqueous methanol 85% as a solvent extraction for 24 h, and fractionated by ethylacetate . The ethyl acetate fractions for the plant were analyzed by high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) for its phenolic acid and flavonoid contents. The antioxidant activity was done on ethyl acetate fraction by DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radical scavenging assay, also this fraction was chosen to test the anti-proliferative action of against breast cancer cell line MCF-7 and rate embryonic fibroblasts (REF) normal cell line, Results: The different chromatographic results revealed the presence of different phenolic compounds in the plant extract like luteolin, rutin, astragalin, isoquercetin, catechins, and 3,5-dicaffeoylquinic acid (3,5-DCQA) and the data showed that ethyl acetate fraction has significant anti-proliferative activity against MCF-7 (IC50 =35. 5µg/ml) and REF (IC50 =1762.9 µg/ml)and possess potent antioxidant activity when compared with reference compound ascorbic acid(Vitamin C). The antioxidant activity may relate to the existence of flavonoids which had been approved as potent antioxidant, and identified by Preliminary qualitative phytochemical analysis.
Keyword: CapparisspinosaL, Flavonoids, Phenolic acid, HPLC, HPTLC, Antioxidant, Breast cancer.
DOI: https://doi.org/10.31838/ijpr/2020.12.02.0158
Download: Request For Article
 




ONLINE SUBMISSION
USER LOGIN


Username
Password
Login | Register
News & Events
SCImago Journal & Country Rank

Terms and Conditions
Disclaimer
Refund Policy
Instrucations for Subscribers
Privacy Policy

Copyrights Form

0.12
2018CiteScore
 
8th percentile
Powered by  Scopus
Google Scholar

hit counters free