|
Effect of Prenatal Ketamine Exposure on ZO-1 Marker Expression in Mice Prefrontal Cortex
|
|
| Author:
|
MUNQITH MGHAMIS, DR. HAYDER J. KADHIM, DR. HAYDER H. ABDULAMEER
|
| Abstract:
|
This study aims to investigate the immunohistochemical changes in ZO-1 antibody in neonatal mice prefrontal
cortex after injection of mothers with anesthetic doses of ketamine during different periods of pregnancy. A
total of 60 adult female and 15 male mice (Swiss albino mice, Mus musculus) 8-12 weeks of age and weighing
25-40 gm. In this study 60 pregnant mice were divided into three groups (20 mice in each group). Each group
was divided into two equal subgroups; control and experimental. The control groups were injected with
distilled water intraperitoneally six times a week at the first, second and third week of pregnancy respectively.
The experimental groups were injected intraperitoneally with anesthetic dose of ketamine hydrochloride (50
mg/kg) during the first, second and third week of pregnancy respectively. Each group was injected six times at
24 hr. interval at day 1, 2, 3, 4, 5 and 6 of first, second and third week respectively using a 30-gauge needle.
Mice neonates were sacrificed by decapitation at age of one day. Routine histological tissue processing was
done, immunohistochemistry was done for detection of ZO-1 expression. Results of group 1 showed the
fluorescent reactivity appeared higher at the area of external granular layer, group 2 showed even distribution
of the fluorescent reactivity all over the section. Immunohistochemical staining of this group showed higher
fluorescent reactivity to Anti-ZO1 fluorescent marker than control. The conclusion made from the ZO-1 IHC
reactivity of this study regarding the disturbances of the newborn ZO-1 protein building in the gap junctions of
the primitive immature neurons as a feature of defected synaptogenesis.
|
| Keyword:
|
Prenatal Ketamine, ZO-1 Marker, Prefrontal Cortex, Fluorescent Immunohistochemistry.
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2020.12.02.0149
|
| Download:
|
Request For Article
|
|
|