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Molecular Study on Diarrheal Bacteria of Goats in Baghdad
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| Author:
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ZENA ALI, SHIRIN JAZAR AMIN, ASIF HASAN ABDULRAZAQ, MOHAMMAD HAMID KAREEM, MUSTAFA SALAH HASAN
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| Abstract:
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Gastroenteritis with signs of diarrhea is one of the major problems facing goat production. The most
commonly incriminated bacterial causes of gastroenteritis and diarrhea in goat is enterotoxigenic E.coli and
C.perfringens. This study aimed for assessing the value related to utilizing biotechnology for quickly detecting
E.coli virulence genes and C.perfringens toxins. Our study revealed that E.coli has been the most common in
the diarrhoeic goat, succeeded via C.perfringens as well as combined infection. E.coli was identified through
isolation on MacConkey`s, EMB and blood agar media, biochemical tests and API 20E protocol. Serotyping of
E.coli isolates indicated that the E.coli O26, O44 as well as O55 were most prevalent serogroups recovered
from diarrheic goat, other serogroups as O125, O151, O1 and O146 were also included. Multiplex PCR assay
succeeded to detect 5 virulence genes of E.coli (stx2, astA, aggR, invE and eaeA genes) with characteristic
bands at 474, 106, 254, 382 and 454 bp, respectively. astA gene was the most prevalent virulence gene among
E.coli isolates. C.perfringens was identified through isolation on goat blood agar with the neomycin sulphate,
biochemical tests as well as the toxin antitoxin neutralization test. Typing related to the C.perfringens isolates
through dermonecrotic tests in the albino guinea pigs indicated that the C.perfringens types A, B, D as well as
the non-toxigenic strains. Furthermore, the multiplex PCR specified that the genes coding for a, ß and e toxins
of C.perfringens isolates with characteristic bands at 324, 196 and 655 bp, respectively.
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| Keyword:
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E. coli, C. perfringens, Goats, Diarrhea
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.02.0145
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| Download:
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