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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Solvent Polarity Based Microwave-Assisted Extraction of Curcuminoids from Curcuma longa L. Rhizome and Their Quantitative Determination by HPLC-PDA Method

Author: UPLOADED BY-ADMIN, SUBASH C. VERMA, C. L. JAIN
Abstract: We reported a study on selection of solvent for microwave-assisted extraction of curcuminoids from Curcuma longa L. rhizome and their quantitative determination by HPLC-PDA method. The main colored substances in the rhizomes are curcumin, demethoxycurcumin and bis-demethoxycurcumin. A variety of solvents ranging from non-polar to polar were used for microwave-assisted extraction of curcuminoids from Curcuma longa rhizomes. 45 ml of acetone as a solvent, microwave power (700 W) and microwave’s irradiation time 180 second were found to be most favorable for maximum extraction of curcuminoids. HPLC experiments were performed on C18 column and acetonitrile: methanol (75:25, v/v) and water: acetic acid (97:3, v/v) were used as a mobile phase with isocratic mode. The detection wavelength was set 425 nm for the analysis of curcuminoids. Proposed method was validated in term of linearity, detection limit, quantification limit, precision and accuracy. The calibration curve was found linear and regression equation revealed a good linear relationship (r2 =0 .999) between the mass of curcuminoids applied and the peak areas. Recovery was achieved in the range 91.46– 96.51% for curcuminoids. Maximum yield of extract (15.57%) was found in methanol: water (60:40, v/v), while lowest extract yield was found with n-hexane solvent (1. 63%). Acetone extract showed maximum yield of curcuminoids (97.51%) on dry weight basis. In this study an efforts was put to fix the standards for commonly used herbal formulations containing curcuminoids, which can be utilized in quality control of the formulations.
Keyword: Microwave-assisted extraction, curcuma longa L. rhizome, curcuminoids, HPLC-PDA
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