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Local delivery of Tacrolimus from Liposomal Gel for treatment of Atopic Dermatitis
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| Author:
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, DEEPA H. PATEL*, HETAL D. KOLADIYA, DIPALI R. TALELE
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| Abstract:
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Atopic dermatitis is chronically relapsing eczematous skin disorder. Tacrolimus inhibits T-cell activation resulting in suppression of inflammation. The common side effects of tacrolimus are burning sensation and pruritus at the application site. The aim of the present investigation was to prepare and characterize FK506 loaded liposomal gel that could successfully target tacrolimus to action site without producing undesirable side effects. Thin layer film hydration method was used to prepare liposomes using HSPC, cholesterol and cationic lipid DOTAP which was later incorporated into HPMCK100M (3%) to formulate the gel and evaluated for viscosity, spreadability, pH, in-vitro drug release, and stability studies. The vesicle size, zeta potential and drug entrapment was found to be 114.6±0.53 nm, 18.5± 0.21mV and 72.36± 0.96% respectively. The pH and viscosity of liposomal gel were found to be 5.6 and 55± 0.023Pa/s respectively with good spreadability. The in-vitro drug release at 24 hr was found to be 80.3±0.013%. Animal studies were carried out on allergic contact dermatitis model in mice exhibited comparatively higher activity with 0.03% tacrolimus liposomal gel than 0.03% marketed ointment. From the results, it was concluded that tacrolimus was successfully prepared as liposomal gel having increased activity than the marketed ointment.
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| Keyword:
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Liposomes, Tacrolimus, Thin layer film hydration, Allergic contact dermatitis, Local action
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| EOI:
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| DOI:
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