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Antidiabetic activity (In vitro alpha amylase inhibitory) of ethanol extract of Carissa carandas Linn. roots.
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| Author:
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SONIA SINGH
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| Abstract:
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Objective: The present research was designed to determine in vitro a-amylase inhibitory activity of ethanol extract of Carissa carandas Linn. root against porcine pancreatic amylase enzyme. Materials and Methods: Ethanolic root extract was prepared using soxhlet apparatus. The different concentrations of 20, 40, 60, 80, and 100 µg/mL of ethanolic root extract of C. carandas was dissolved in dimethyl sulfoxide, which was then used for in vitro alpha amylase inhibitory assay. The starch azure was used as substrate. The activity was measured at an absorbance of 595 nm using UV-spectrophotometer. The percentage of inhibition and IC50 value was evaluated and compared with the standard drug, Acarbose. Results: Ethanolic root extract attained potent effects in vitro a-amylase inhibitory activity of 71.16 ±0.01 % at 100 µg/mL with an IC50 value of 41.47 µg/mL, compared with acarbose as standard drug with IC50value of 84.42µg/mL. Conclusion: Ethanol extract of C. carandas Linn. root possessed effective in vitro a-amylase inhibitory property. For further aspects, this current study would be found helpful in the management of life style disorders, viz., diabetes.
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| Keyword:
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acarbose, absorbance, antidiabetic, % inhibition.
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.03.002
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| Download:
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