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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Quantitative Analysis of Microbiota in Patients with Orthopedic Structures on Dental Implants Using the Real-Time PCR Method

Author: AZIM OLIMOV, ARTUR KHAYDAROV
Abstract: Microbiological research was carried out by PCR method in 42 persons aged 25-60 years (men - 24 and women - 18), installed and Osseo integrated DIO dental implants, which were prototyped by metal-ceramic crowns, with temporary fixation on Tempo Bond NE. After 20 days, a follow-up visit was scheduled. All the examined patients were taken biological substrate of gingival fluid from the para-implant furrow using paper endodontic pins (? 25). Exposure of two pins was made within 30 seconds with the help of tweezers between the peri-implant cuff and the crown, biological substrate of gum liquid was taken from the parai-implant furrow. The biological substrate was transported and tested according to the manufacturer's recommendations. When studying the content in patients with orthopedic constructions on dental implants by PCR method we observed high frequency of occurrence of Streptococcus sobrinus (69,3%), Streptococcus sanguis (48,6%) and Porphyromonas gingivalis (41,9). Relatively similar data on the content of these microorganisms were obtained in the molecular genetic study of Streptococcus oralis samples (22.7%). Obviously, it is the combined effect of the most frequently diagnosed pathogens and peculiarities of interaction of anaerobic agents of parasitocenosis that can determine the nature of inflammatory and destructive process in the peri-implant zone.
Keyword: PCR, pathogenic, opportunistic pathogens, internal interface, paraimplant area, implant, abutment.
DOI: https://doi.org/10.31838/ijpr/2020.12.02.0110
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