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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Exploration of Antioxidant Potential of Various Medicinal Plants Beneficial for Cancer Therapeutics and Phytochemical Characterization, Standardization Thereof

Author: SWATI MADAN, SATYENDRA KUMAR RAJPUT, NEERUPMA DHIMAN, BITASTA MANDAL, ROHIT BHARDWAJ
Abstract: Nature is a storehouse of a large number of plant and animal derived materials which are reported to have immense pharmacological potential. The current study is based on the fluorescence analysis, evaluation of physicochemical parameters, preliminary phytochemical screening and analysis of antioxidant potential of the aqueous extracts made from the aerial parts of Catharanthus roseus and stem bark of Camptotheca acuminata. Physicochemical evaluation was performed as per the official guidelines. Fluorescence behavior of the plant material was studied under visible light and UV light at 254 and 366 nm. Phytochemical screening was done as per the literature. Antioxidant activity was assessed using different in vitro models such as DPPH free radical scavenging assay, ABTS radical scavenging assay, ferric reducing antioxidant assay, superoxide anion radical scavenging assay and nitric oxide radical scavenging assay. Ascorbic acid was used as the reference standard. The fluorescence behavior exhibited by both the plant materials was unique to them. Results of physicochemical analysis also complied as per the officially set limits. Preliminary phytochemical screening revealed the presence of tannins, alkaloids, saponin, terpenoids, cardiac glycosides and flavonoids. Both the aqueous extracts exhibited significant antioxidant activity thereby highlighting their tremendous therapeutic efficacy.
Keyword: Catharanthus roseus, Camptotheca acuminata, fluorescence, physicochemical analysis, preliminary phytochemical screening, antioxidant
DOI: https://doi.org/10.31838/ijpr/2020.12.02.0090
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