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Investigating the Effect of Multipotent Mesenchymal Stromal Cells Secretome on Imiquimod-Induced Psoriasis in Rats
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| Author:
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MARINA SARYCHEVA, NATALIA A. NADEZHDINA, SERGEI V. NADEZHDIN, VYACHESLAV P. BONDAREV, ALEXEY V. MYCIC, YURI E. BURDA, MIKHAIL V. POKROVSKIY, LYUDMILA M. DANILENKO, ANNA A. PERESYPKINA
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| Abstract:
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Background: Multipotent mesenchymal stromal cells secretome contains a range of anti-inflammatory factors and can be used for the treatment of psoriasis.
Material and Methods: The study included 16 male Wistar rats weighing from 214 to 256 g, which were divided into 2 subgroups: 8 rats — positive control, treated with fluocinolone acetonide (“Sinaflan” 0.025% ointment, MPZ, Russia), 8 rats - an experimental group that received a cream with a secret bone marrow MSC. Psoriasiform inflammation was caused by daily application of 50 µl of 1% imiquimod suspension (Xi'an Leader Biochemical Engineering Co., Ltd, China) on the skin of the back from the inner and outer surfaces in olive oil with DMSO in a ratio of 9:1 for 4 days. After the development of severe inflammation, treatment was started on both sides: on the one hand, each animal received a baby cream (negative control), on the other hand, fluocinolone or cream with the addition of MSC secretome in the corresponding group, application was carried out 1 time per day for 3 days. On the 8th day of the experiment, the animals were euthanized and skin fragments were collected for histological examination.
Results and Discussion: On the day 4 of the imiquimod use, all animals showed the development of inflammation, with the appearance of coarse lamellae, excoriations, evenly on both sides in the application of the inducer of inflammation. The relief of inflammation as a result of daily single application of cream, cream with MSC secretion and fluocinolone was observed in the experimental group and in the positive control from the 2nd day of use, in the negative control on the 3rd day of the cream application the inflammation decreased slightly.
Conclusion: The efficacy of topical administration of MSC secretome in relieving psoriasiform inflammation in Wistar rats is comparable to that of glucocorticoid with high activity (class 3 according to the European classification of Miller & Munro) fluorocinolone acetate, both by clinical signs and by morphometric indicators.
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| Keyword:
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inflammation, secretome, multipotent mesenchymal stromal cells, imiquimod, psoriasis.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.04.0326
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