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Modeling L-Name Induced Nitric Oxide Deficiency considering the The Cardio- And Endothelial Protective Effects
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| Author:
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PAVEL KOLESNICHENKO, DMITRY V. SHCHEBLYKIN, ALEXEY N. DEMIDENKO, OLESYA V. SHCHEBLYKINA, KONSTANTIN M. REZNIKOV, ALHAMZAH MUHI ALDEEN AZEEZ, MAXIM A. ZHUCHENKO, SERGEY A. DEMCHENKO
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| Abstract:
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Introduction: Currently, endothelial dysfunction is considered as a predictor of a number of pathologies, including arterial hypertension, ischemic heart disease, chronic heart failure, and is also a pathogenetic component of organ damage in diabetes, hypoestrogenic and other conditions.
The purpose of the study is an experimental study of the cardio and vasoprotective effects of etoxidol under conditions of endothelial dysfunction.
Materials and methods: The study was performed on Wistar rats. The pharmacological properties of ethylmethylhydroxypyridine malate (etoxidol) were studied on the model of endothelial dysfunction caused by the blockade of NO synthase on the background of the seven-day administration of L-NAME (25 mg / kg). To evaluate the effect of etoxidol on endothelial function, a calculated index of functional vascular samples — the coefficient of endothelial dysfunction — was used, and the morphological picture of the myocardium (determined the diameter of cardiomyocytes), carotid arteries (intima / media ratio) and adrenal mass was studied.
Results: The seven-day administration of L-NAME led to the development of severe endothelial dysfunction. The use of etoxidol in a dose of 50 mg / kg against a background of relatively high blood pressure values reduced QED by 30%, and by 49% when using a dose of 90 mg / kg. With a relatively high diameter of cardiomyocytes in all studied groups receiving L-NAME, a significant (p=0.05) difference with the control was obtained with a dose of 90 mg / kg. When studying the intima / media ratio in the carotid artery, a significant decrease in the ratio to the control was obtained in the group receiving etoxidol at a dose of 90 mg / kg.
Conclusion: etoxidol has a pronounced endothelial-, vaso- and cardioprotective activity. The effectiveness of the drug is dose-dependent: the best endothelium and cardioprotective effects are achieved with a dose of 90 mg / kg.
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| Keyword:
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ethylmethylhydroxypyridine malate, L-NAME, endothelial dysfunction, cardioprotection.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.04.0320
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