|
Change in CRT gene sequence of Plasmodium falsiparum as a chloroquen resistant in Iraqi patients
|
|
| Author:
|
ABDULRAZZAQ ZGHAIR, NAZAR SH. MOHAMMED, SALWA S. MUHSIN
|
| Abstract:
|
This study was conducted on (20) individuals whose ages ranged between (9-15) years and suspected to be infected with malaria in Qanaqin and Al-Saadiya towns / Diala province in Iraq during the period from1st June 2018 to 15th may 2019. The results showed that 9 (45%) of them were positive for plasmodium falciparum by using ELISA and IFAT techniques. Conventional PCR was used to determine the genotypes of the parasite. The primers used included forward primer ACTCTGTAGTTTGTAGAGATGCAA and reverse primer ACGGCACATTATCTCACCGTT, in the product of 504 length. Mutations occurred in Iraqi patients infected with chloroquine resistance (CRT) P. falciparum genes. The AAA changed to ATA and AAA to ACA respectively.
|
| Keyword:
|
Plasmodium falsiparum, CRT gene, chloroquine-resistant
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2020.12.02.0069
|
| Download:
|
Request For Article
|
|
|