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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence

IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Enzyme activity and estimate the quantitative degradation of potential bacterial isolates from Dairy and Bulk drug industries effluent with respective substrates

Author: FARQAD HWAIDI, PANDU. BRAHMAJI RAO
Abstract: This study was done to study the effect of Pseudomonas ES10 and VDLL5, Stenotrophomonas HDUL4 and VDLL1 strains isolated from dairy and bulk drug industry water samples to produce enzymes and to degrade crude oil, phenol and heavy metals. From the selected bacterial strains, only HDUL4 produced all enzymes except lipase. VDLL1 strain produced protease and cellulose, and no lipase and amylase production. ES10 and VDLL5 strains showed all enzymes production at optimal conditions. Among the enzyme activities, HDUL4 showed low Amylolytic activity (3.98U/ml/mim) and ES10 showed highest (12.73 U/min/ml). In cellulolytic activity VDLL1 strain showed less and ES10 showed more activity. Among the strains HDUL4 showed less and ES10 showed more Lipolytic activity. HDUL4 showed low Proteolytic activity (2.38 U/min/ml) and ES10 showed highest (9.89 U/min/ml). ES10 strain showed maximum (70%) and VDLL1 showed minimum (30%) phenol degradation. The results showed that the bacterial strains are effective in biodegradation of oil, phenol and heavy metals as well as enzyme production.Also these identified strains are good at production of degradative enzymes.
Keyword: Pseudomonas, Stenotrophomonas, oil degradation, phenol degradation, heavy metal degradation, Enzyme activity
DOI: https://doi.org/10.31838/ijpr/2020.12.02.0078
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