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Association of Trichomonas Vaginalis and E. coli bacteria in vaginal infections with drug resistance isolates
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| Author:
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NAZAR MOHAMMED, YAQOOB Y. ISMAIL, AYA R. ABDULWAHHAB, ABDULRAZZAQ NEAMAH ZGHAIR
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| Abstract:
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Trichomonas Vaginalis infection may lead to complicated infections in association with E. coli bacteria. To detect Trichomonas Vaginalis gene by amplifying sized pieces 318bp from MLST gene, the DNA extracted PCR by deport is deported electrically in the reaction solution with 1.5% concentration of agarose gel by using DNA size ladder 100bp. Mutation occurred in 441, 449. 459, 886 and 990 positions of MLST gene respectively. In this locus, the allele was changed (T to C) at position 81244bp, and showed one silent mutation in allele C14179T, (G 871 A) that appeared in two isolates belonging to secondary infertile patients who had cervical abnormalities but not in other patients. For the two closely-related K-12 strains, W3110 & MG1655, highly accurate genomes were determined so as to solve the whole-cell problem with Escherichia coli K-12 as a cell model. Completing W3110 genome and comparing it with MG1655 genome demonstrated variations in 267 sites, including 251 sites with short, mostly single-nucleotide deletions or insertions base substitutions of (total 358 nucleotides).
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| Keyword:
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Trichomonas vaginalis , E. coli, gene mutation, vaginal infections
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.02.0077
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| Download:
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