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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Investigation of Response to Antiretroviral Therapy and Genotyping of Human Immuno- Deficiency Virus in A Sample of HIV- Positive Patients in Iraq

Abstract: Aim of study: assessing the response to anti-retroviral regimen taken in Iraq by measuring HIV plasma viral load, and host CD-4 count prior and post therapy. Patients and methods: A cross sectional study that was conducted in the Department of Public Health / Central Public Health Laboratory during the period from October 2018 to May 2019. included 25 HIV-positive patients at any age or gender. HIV patients who had already at the final AIDS stage of the disease were excluded from the study. Data were collected through direct interview with the patients using a questionnaire. HIV patients before therapy were subjected to rapid test, ELISA, and minividas test for confirming HIV infection and the values of viral load and CD4 were recorded from patients’ registries. After anti-retroviral therapy, the patients were subjected to quantitative RT-PCR for measuring plasma viral RNA load, and CD4 count. Results: Mean of viral load was significantly decreased while mean of CD4 was significantly increased after treatment compared to that before treatment. Mean of viral load was significantly (P < 0.05) decreased and mean of CD4 count after treatment compared to that before treatment in patients aged < 40 or = 40 age groups; in male gender; in those with duration from exposure to treatment < 8 or = 8 months; in patients treated by triple (viraday), quadruple (genvoya), or bilateral (vidax); and in patients treated for < 12, or = 24 months. Conclusion: HIV therapy generally was shown to significantly lower plasma viral load and raise CD4 count. Quadruple therapy was shown to be the best among other types in reducing viral load and enhancing CD4 count.
Keyword: HIV, Viral load, CD4 count, anti-retroviral therapy, Iraq
DOI: https://doi.org/10.31838/ijpr/2020.12.02.0034
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