IJPR  articles are Indexed in SCOPUSClick Here     Impact Factor for Five Years is 0.13 (2013 - 2018).    

logo

INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence

IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
Current Issue
Article In Press
RMDL: Classification of Parkinson's disease by nature-inspired Algorithm
Antidiabetic activity (In vitro alpha amylase inhibitory) of ethanol extract of Carissa carandas Linn. roots.
Six sigma: an embellished exploration in the field of pharmaceutical industry
The effect of Sida acuta on bacterial enzymes in azoxymethane-induced experimental colon cancer
Premature ageing in children: a rare genetic disorder called progeria.
Stress, Depression & Gut Microbiota: The Gut-Brain Axis Regulation
Zinc oxide nanoparticles and antibiotics mediated combinatorial approach to enhance antibacterial potential
Quality of life and associated factors among students in Jambi Province, Indonesia
Novel method for simultaneous determination of spiramycin and metronidazole in pharmaceutical dosage form by UV-visible absorption spectrophotometry
The Influence of Physicians Social Capital on Patients Satisfaction
1234
ADOBE READER

(Require Adobe Acrobat Reader to open, If you don't have Adobe Acrobat Reader)

Index Page 1
Click here to Download
IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

Click to download
 

Article Detail

Label
Label
Diagnosis of Toxoplasmosis in Pregnant Women Using Enzyme Immunoassay and Nested Real Time PCR in Al-Najaf city/Iraq

Author: MUNA GHAZZAY, KHALIDA K ABBAS AL-KELABY, EMAD SADIQ ALI AL-HILLI, NAJAH R. HADI
Abstract: This work was planned as diagnostic study to evaluate the prevalence of Toxoplasma Gondi (T. Gondi) among the population of 146 pregnant women of ages ranging from 14-45 years old, during the period from 20th of October 2018 to 14th of January 2019 at Al Najaf Public Health laboratory, and 50 normal pregnant women (control group), these cases were distributed according to the age groups, residency and IgM or IgG seropositivity. The diagnostic assay was firstly depending on Enzyme immunoassay (EIA) for the evaluation the prevalence of anti- T. Gondi IgM and IgG antibodies in the sera of pregnant women, then confirmed by the molecular technique (Real-time PCR) using specific primers for the detection of B1 gene of T. Gondi. This detection was also followed by confirmatory nested polymerase chain reaction using RE gene. Results presented in this study showed that overall seroprevalence of toxoplasmosis were 55:146 (37.67%). Out of 146 women; 53(36.3%) of samples had chronic toxoplasmosis characterized by the presence of positive IgG antibodies, while only 2 (1.37%) were positive for acute toxoplasmosis characterized by the presence of positive IgM antibodies. The prevalence of abortion according to age groups revealed that 34.9% (51:146) of the total pregnant cases; 17:53 (32.08%) of chronic T. Gondi infection and the two positive acute cases (2:51) of T. Gondi infection were related to women of 20-25 years age group. While 30.19% of toxoplasmosis seropositivity (16:53) were related to 32-37 years age group. According to the residency, 86:146(58.91%) were from rural area, while 60:146 (41.09%) were from urban area in Al-Najaf. Results also showed that out of 55 T. Gondi seropositivity cases, 39 (70.9%) were living in urban area and 16 (29.01%) were living in rural area. About 71.69% of IgG seropositivity patients were lived in urban area and about 28.31% were lived in rural area. Data regarding the two IgM positive cases showed that the infection distributed evenly (50%) for both rural and urban areas in Al-Najaf. Results were also showed statistically non-significant differences in accordance to the hospitalization need Control group investigation showed that 7:50 (14%) cases were seropositivity for anti- T. Gondi IgG, while all control cases revealed IgM seronegativity. The statistical analysis of PCR data of T. Gondi threshold cycle (CT) of B1 and RE genes were revealing mean ±Standard deviation of 18.6± 5.2 and 13.1± 1.3 respectively. The interassay coefficient of variation (CV) of B1 and RE genes of T. Gondi was 28% and 10.3% respectively. We concluded that B1 and RE genes detection was successful, confirmatory to EIA and more précised for T. Gondi isolated from serum samples.
Keyword: Toxoplasmosis, B1 gene, RE sequence, Nested PCR.
DOI: https://doi.org/10.31838/ijpr/2020.12.02.0022
Download: Request For Article
 












ONLINE SUBMISSION
USER LOGIN


Username
Password
Login | Register
News & Events

Terms and Conditions
Disclaimer
Refund Policy
Instrucations for Subscribers
Privacy Policy

Copyrights Form

0.12
2018CiteScore
 
8th percentile
Powered by  Scopus
Google Scholar

hit counters free