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Nephroprotective potential effect of U-50488H in renal ischemia reperfusion injury in adults Males rats’ model: Role of NRF2 pathway
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| Author:
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MARYAM ALAWADI, MUNTHER ABOSAOODA, RASHA HATEM DOSH, MAYSAA ALI ABDUL KALEQ, NAJAH R HADI, WADDAH MAHBOBA
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| Abstract:
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Renal ischemia reperfusion injury promotes tissue damage through inducing uncontrolled inflammation, oxidative stress and excessive renal tubular epithelial cell death. Renal ischemia reperfusion injury is one of the main causes of acute kidney injury, it occurs in various clinical situations such as partial nephrectomy, renal transplantation, cardiopulmonary resuscitation, sepsis and shock. Nuclear factor Erythroid 2-related factor 2 pathways are essential in protecting the kidney against renal ischemia reperfusion injury. U-50488H is a highly selective Kappa-opioid receptor agonist. It has anti-inflammatory effect. The present study aims to study the nephro-protective potential effect of U-50488H in renal ischemia reperfusion injury in rats’ model via modulation of Nrf2 pathway. At the end of the experiment, in Control group, as compared with Sham group, the renal tissue levels of Nrf2, HO-1, P-Akt, NF-kB, IL-1ß, F2-isoprostane, NGAL and levels of blood Urea and serum Creatinine were significantly (p?0.05) increased, in addition, Control group causes a significant histopathological tissue damage (p?0.05). Pretreatment with U-50488H exert protective role in the kidney against injury by a significant (p?0.05) more increase in the tissue level of Nrf2, HO-1, P-Akt and decrease in the tissue levels of IL-1ß, NF-kB, F2-isoprostane, NGAL and levels of blood Urea and serum Creatinine. Furthermore, U-50488H a significantly (p?0.05) reverse the severity score of renal tissue damage. From the overall results, we conclude that U-50488H a significantly decrease renal ischemia reperfusion injury in adult male rats model through Nrf2 pathway via it is pleiotropic effects as anti-oxidant, anti-inflammatory and anti-apoptotic.
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| Keyword:
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U-50488H in Renal Ischemia/Reperfusion Injury
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.02.0021
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