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Antioxidant Activities and Identification of Compounds in Ethyl Acetate Fraction, Dichloromethane Fraction And Ethanol Extract Of Garcinia mangostana L. Leaves
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| Author:
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DINIATIK , ANIS KUSUMAWATI, AGUS SISWANTO, DIANA MARTALIA DOTI
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| Abstract:
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Mangosteen leaves (Garcinia mangostana L.) are a good source of antioxidants. The compounds contained therein are flavonoids and xanthone. This study aims to compare antioxidant activity and identify active compounds and to determine the types of flavonoids from ethanol extract, dichloromethane fraction and ethyl acetate fraction of G. mangostana leaves. The maceration method was used as extraction with solvent 70% ethanol. The thick extract was hydrolyzed using 2 N hydrochloric acid, then partitioned with dichloromethane and ethyl acetate. Ethanol extract, dichloromethane fraction, and ethyl acetate fraction were tested for antioxidant activity using DPPH method with UV-Vis spectrophotometer and quercetin as standard. The IC50 value of ethanol extract, dichloromethane fractions and ethyl acetate fraction were 0.819 µg/mL, 0.723 µg/mL, 0.025 µg/mL. Flavonol Quercetin as a standard has IC 50 of 0.131 µg/mL. It indicates that the extract and fraction of G. mangostana leaves have potent antioxidant activity and ethyl acetate fraction has higher antioxidant activity than other solvents.
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| Keyword:
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Garcinia mangostana L, antioxidant activity, ethyl acetate fraction, ethanol extract
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.02.0010
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| Download:
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