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RP – HPLC Developed Method for paracetamol, Caffeine and Chlorpheniramine Maleate Estimation in Pure and pharmaceutical formulation
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| Author:
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IQBAL MOHAMMED, DUAA A. YASS, INAM H. KUDHAIR
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| Abstract:
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A sensitive, efficient, reproducible and rapid method for the specific determination of Paracetamol(PARA), Caffeine
(CAF) and ChlorpheniramineMaleate(CHP) as pure and in pharmaceutical formulation has been developed using
reverse phase high performance liquid chromatographic (RP - HPLC). This method involves separation of the drugs
on reverse phase HPLC Shimadzu LC–20 A, Japan and Phenomenex C18 column (250 × 4.6 mm, 5µm). The
elution was done using a mobile phase consisting of sodium acetate buffer: acetonitrile (ACN) as the ratio of
(90:10 v/v with pH adjusted at 3.0 using acetic acid). The separation was monitored for 10 min at a wavelength of
244nm,272nm and 262 nm of the Paracetamol, Caffeine and ChlorpheniramineMaleate respectively using a UVvisible
detector and 1.2 mL/min flow rate. The optimum conditions such as flow rate, the compounds of the
mobile phase, wavelength and pH were studied. The obtained results revealed that the value of R2 is (R² = 0.9993)
for Paracetamol, (R² = 0.9993) for Caffeine and (R² = 0.9978) the LOD (µg/mL) was (0.1) for Paracetamol,
Caffeine and (0.6) for ChlorpheniramineMaleate while LOQ (µg/mL) was (0.3) for Paracetamol, Caffeine and (1.65)
for ChlorpheniramineMaleate, the linear ranges (1 – 25) µg / mL. The recoveries of standard Paracetamol in were
between 98.8 – 100.7%, Caffeine 96.0 – 102.2% and ChlorpheniramineMaleate 96.4 – 100.05% Compared to the
other methods; the current method is rapid, simple and economical for the determination ofPARA, CAF and CHP.
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| Keyword:
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RP-HPLC, Sensitive, Developed, Paracetamol, Caffeine and Chlorpheniramine Maleate
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.01.135
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| Download:
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