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Effects of topical Phenytoin,Chitosan, Dextrin, and Chitosan-Dextrin Combinations in Experimentallyinduced Thermal Injury in Rabbits
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| Author:
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HMED ABD, ABDULKAREEM H. ABD, MUHAMMED A. H. ALDABAGH
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| Abstract:
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Phenytoin has been known to promote wound healing like burns injury because it owing to its proliferative as well
as anti-inflammatory effects. the aim of this study to evaluate the effects of topical chitosan 2% cream
(Ch
), alone
and in combination with Phenytoin
(Phenytoin 1%+Chitosan 1%)cream combination
(PhCh
)on experimentally-
induced thermal injury in albino rabbits. Seventy two of albino male rabbits, weighing 1250-1750 g were divided
into six groups, each of them 12 animals: AH group: apparently healthy rabbits, BWT group: treated with base
Aqua Rosa cream, PhCh group: treated with phenytoin 1% ,chitosan 1% and dextrin prepared cream, Ch group:
treated with chitosan 2%- dextrin,SSD group: treated with silver sulfadiazine cream
(control
); all animals
(except
AH group
) were induced burn and treated topically on burned area twice daily for 28days. Tissue
levels of
vascular endothelial cell growth factor
(VEGF
),transforming growth factor beta
(TGF-ß
), and skin histopathological
examination were measured on days 7, 14, 21, and 28 of burn injury experiment. Histopathologic evaluations at
the day 7 showed that neovascularization, granulation tissue and collagen scores were greater in the PhCh group
than the Ch, SSD and BWT groups. On day 21, re-epithelialization scores were showed higher and maximum level
in the PhCh group than the Ch, SSD , and BWT groups. Inflammatory cells scores in BWT,SSD, and Ch groups
showed higher than PhCh group . PhCh, Ch, and SSD groups have a significant increases in VEGF levels in
compared with AH and BWT groups.Ch and SSD groups showed distinctive elevation in TGF-ß level while PhCh
group still within normal level during sampling itervals.
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| Keyword:
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Phenytoin,vascular endothelial cell growth factor
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.01.061
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| Download:
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