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Standardization of a Crude Drug Moringa oleifera Leaf from Africa, India and Local (Indonesian) which Cultivated in Bojonegoro Indonesia
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| Author:
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NIKMATUL EKA, KARTINI, LINTANG KARINA PUTRI
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| Abstract:
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Purpose: The research was to determine specific parameters (macroscopy and microscopy) and non-specific
parameters (loss on drying, total ash content, acid insoluble ash content, water-soluble extractive matter,
ethanol-soluble extractive matter), heavy metal contaminant (Pb, Hg, As, Cu) and microbial contaminant (Total
Plate Count and Total Yeast Mold). Research also determined a total flavonoid of M. oleifera leaf.
Methodology: The method carried out according to the procedure stated in the Materia Medika Indonesia
5
th
edition.
Methods to detection Contaminants were referred to WHO guidelines for assessing the quality of herbal
medicines. The determination of total flavonoid was performed by spectroscopic method.
Results: Crude drug of M. oleifera leaf meet the specific parameters (macroscopy and microscopy). Nonspecific
parameters
of
Africa,
India,
Local
M.
oleifera
leaf
shows
that
loss
on
drying
(8.06
±
0.03;
8.89
±
0.31;
7.56
±
0.17)
%,
total
ash
content
(8.64
±
0.43;
10.64
±
0.90;
15.31
±
0.87)
%,
acid
insoluble
ash
content
(0.56
±
0.08;
0.35
±
0.01;
0.36
±
0.06)%,
water-soluble
extractive
matter
(21.38
±
1.39;
30.12
±
2.06;
12.68
±
1.12)
%,
ethanol-soluble
extractive
matter
(39.37
±
1.51;
27.74
±
2.44;
27.09
±
1.43)%,
contaminant
test
including
heavy
metal
contaminant
shows
that
Pb,
Cd,
As
and
Hg
were
not
to
be
detected.
Microbial
contaminant
(Total
Plate
Count
and
Total
Yeast
Mold)
under
limits
of
WHO
standart.
Total
flavonoid
content
of
Africa,
India,
Local
M.
oleifera
leaf
were
8.12
±
0.52
mg/
100
mg
QE;
10.69
±
0.15
mg/
100
mg
QE
dan
13.08
±
0.08
mg/
100
mg
QE
respectively.
Conclusion:
Moringa Leaves meet specific parameters (macroscopy and microscopy test). Non-specific
parameter tests of Africa, India, Local Moringa Leaf shows that loss on drying, acid insoluble ash content,
water-soluble extractive matter, ethanol-soluble extractive matter, contaminant test including heavy metal
contaminant shows that (Pb, Cd, As and Hg) and microbial contaminant (Total Plate Count and Total Yeast
Mold) meet the standard required, only total ash content did not meet the standard. Total Flavonoid content
shows that the extract of M. oleifera from local (Indonesia) variety is higher than others.
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| Keyword:
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M. oleifera leaf, Standardization, Crude Drug
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.01.134
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| Download:
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