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A Study on Antimycobacterial Activity and Phytochemical Constituents of Dipterocarpus sublamellatus foxw. Hexane Extract (DSHE)
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| Author:
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SITI SABRAN, MUHAMAD HARITH MAZLUN, ZUNOLIZA ABADULLAH, MARYATI MOHAMED, MOHD. FADZELLY ABU BAKAR, FURZANI PA’EE, ALONA CUEVAS LINATOC
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| Abstract:
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Purpose: To investigate the antimycobacterial activity and to profile the phytochemicals of Dipterocarpus
sublamellatus hexane extract (DSHE).
Methodology: Using Mycobacterium smegmatis as a rapid screening model of TB strain, the bacterial cells were
treated with 4-fold of Minimum Inhibitory Concentration (MIC=0.78 mg/mL) of DSHE. Significant cellular leakage
of DSHE-treated cells were quantified spectrophotometrically at 280 nm. Microscopic study using FE-SEM was
employed to indicate any morphological changes induced on bacterial cell shape. Phytochemical profiles of DSHE
was conducted using GC-MS analysis.
Results: The bacterial cells treated with 4-fold of Minimum Inhibitory Concentration (MIC=0.78 mg/mL) of
DSHE rapidly inhibited the bacterial cell growth as early as 8 h of treatment. cell membrane integrity were
severely interrupted and losing the vital constituents for cell survival and growth. Substantial morphological
changes were induced on bacterial cell shape, supporting the interaction of DSHE with cell wall/membrane of M.
smegmatis. GC-MS analysis of DSHE suggested that pyrazole and phenanthroline derivatives were among 23
major phytochemical constituents that potentially contributed to the antimycobacterial activity.
Applications/Originality/Value: A further study in this direction potentially leads to the discovery of targetspecific
antimycobacterial
agents
and
provides
a
basis
that
this
timber
species
has
to
be
conserved
and
managed
sustainably.
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| Keyword:
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Anti-Mycobacterial Activity, DSHE, GC-MS analysis
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.01.133
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| Download:
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