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Investigation of Immunomodulatory Active Compounds from Faloak (Sterculia quadrifida R.Br.) Bark Fractions
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| Author:
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MUNAWAROH, R., SISWADI , SETYOWATI, E.P., MURWANTI, R. , HERTIANI, T.
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| Abstract:
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Faloak (Sterculia quadrifida) bark is an indigenous plant that is used traditionally to restore stamina in East Nusa
Tenggara, Indonesia. This research aimed to investigate the immunomodulatory active compounds from faloak
bark fractions. The faloak bark ethanolic extract dissolved in methanol:water (9:1v/v) then subsequently
partitioned with n-hexane, ethyl acetate, and water. N-hexane and ethyl acetate fractions were separated using
Vacuum Liquid Chromatography of which each yielded six fractions. Those fractions (125 µg/mL) were tested
for their effect on peritoneal macrophages phagocytosis of Balb/c mice in vitro using the latex beads method.
Phagocytosis capacity was analyzed using one-way ANOVA and post hoc Tukey HSD test with a 95%
confidence level. Components of the active fractions were identified using Thin Layer Chromatography and
further analyzed using HPLC-ESI-MS/MS. The multiple reaction monitoring (MRM) detection was operated in
the negative ion electrospray ionization (ESI) mode using the transitions of m/z 190.90 ([M ? H]¯ ) ?176.00;
m/z 190.90 ?148.00; m/z 190.90 ? 104.05 for scopoletin. Results of the immunomodulatory assay showed
that the most potent fractions were not significantly different from the activity of the positive control used at
125 µg/mL (a commercial product containing echinacea and black elderberry fruit extract) (p<0.05). The active
fractions were H5 (from n-hexane fraction) and E3 (from ethyl acetate fraction). Scopoletin was found in H5
and E3 by TLC and LCMS analyses. Scopoletin is expected as the active ingredient responsible for increasing
macrophage phagocytosis activity.
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| Keyword:
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Sterculia quadrifida, faloak bark, macrophage phagocytosis, scopoletin
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.01.111
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| Download:
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