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The effect of environmental temperature on the mammary cancer cells and the infiltrating lymphocytes
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| Author:
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KUSMARDI KUSMARDI, YULI FELISTIA , PUSPITA EKA WUYUNG, RIA KODARIAH, DEXTRA F DZAKIROSIN
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| Abstract:
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Purpose: To identify whether temperature has effect on proliferation in the tumor cell (adenocarcinoma) especially
in vivo condition
Methodology: The experiment was conducted in true experimental design (parallel), in which C3H mice are used,
arranged in three cages with different temperatures: 20-22°C, 25-27°C, and 32-34°C in 2 weeks every day for 6
hours each day. The proliferation of the cells was detected with Argyrophilic Nucleolar Organizer Region
(AgNOR) staining (mAgNOR and pAgNOR) and lymphocytes were stained with Hematoxylin-Eosin staining.
Results: As the result, One-Way ANOVA analysis shows p = 0,000 for mAgNOR and p = 0.000 for pAgNOR. In
addition, Mann-Whitney analysis for lymphocyte counts shows p = 0.0241 for both 20-22°C & 25-27°C and 2527°C
&
32-34°C
Applications/Originality/Value:
The temperature affects cell proliferation in vivo. and number of tumor ilfiltrating
lymphocytes.
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| Keyword:
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AgNOR
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| EOI:
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| DOI:
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