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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Preliminary Detection, Isolation and Characterization of Astragalin and Amentoflavone from Viburnum Linn.

Author: UPLOADED BY-ADMIN, SPECIES. K PRABHU, P K KARAR, S HEMALATHA, K PONNUDURAI, DEEPAK SINGH
Abstract: The genus Viburnum Linn. under the family-Caprifoliaceae (formerly) and Adoxaceae (currently) consists of about 200 species throughout the world. Many of these species have been reported to possess phenolic compounds and their glycosides, in addition to sterols and triterpenes, as their major chemical constituents in the stem and root barks. These chemical constituents may be attributed to their remarkable biological activities against smooth muscle related troubles. The main objective of the present study is to detect, isolate and characterize some phenolic compounds from aqueous ethanolic stem extract of two Viburnum Linn. species, collected from Nilgiri hills, Tamilnadu, India. The dried stems of Viburnum punctatum Buch.- Ham.ex D.Don and Viburnum coriaceum Blume. were powdered and extracted separately, successively with petroleum ether (60 – 80°C), chloroform, 75% aqueous ethanol. The ethanolic extracts were screened by Thin Layer Chromatographic techniques for the presence of different classes of phenolic compounds, since the glycosides of phenolic compounds such as flavones, flavonols, biflavone and phenolic compounds of C6-C3 are appreciably soluble in alcohol rather than the other two fractions. The TLC studies revealed the presence of some flavonoids which was later confirmed by spectroscopic analysis and melting point to be astragalin in V.punctatum and amentoflavone in V.coriaceum. The ethanolic residues of V.punctatum and V.coriaceum were adsorbed on the activated neutral alumina and packed in a glass column containing slurry of alumina in petroleum ether. Then, the column was eluted firstly with some non-polar and moderately polar solvents to clear out impurities, which remain intact in the column. Then, the content of the column was subjected to a gradient elution with methanol- water mixture of decreasing polarity. The fractions (46-55) by 60% and 80% methanol yielded about 75 mg of astragalin from V.punctatum and the fractions (36-46) by 90% and 99.8% methanol yielded about 35 mg of amentoflavone from V.coriaceum. The samples were also characterized by IR, NMR and Mass spectral studies for further confirmation, in addition to their melting point and homogeneity determination.
Keyword: Viburnum, Adoxaceae, gradient elution, astragalin, amentoflavone
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