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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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Prevalence and Risk Factors of Occupation Induced Asthma among Traffic Police in Bangalore City
o Estimate the Prevalence of Depression, Anxiety, and Stress among Adolescents in Kancheepuram District, Tamil Nadu
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An evaluation of Epidemiological Factors Relating to Overweight, Obesity in Urban Chennai
A study on the Pattern of Body Image Perception among Adolescents in Urban Chennai
mpact of an External and Internal factors of overweight and obesity in urban
A study on Anti-Rabies Vaccine among Dog Bite Victims in chennai region
Hospital Based Cross Sectional Study: Distribution of Compliance for completion of ARV 4 dose among study participants
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Evaluation of anticancer potential for L-glutaminase purified from Bacillus subtilis

Author: ASMAA HUSSEIN, SHYMAA I.ALBARAZANCHI , AHMED F.AL-SHANON
Abstract: In past decade cancer therapy researchgavea promissing forward and many facts suggest thepotenteffects of enzymes depleting amino acids in decreasing the risk of various cancers. In this study L-glutaminase as one of the medically important enzymes purified from Bacillus subtilisusing80% ammonium sulfate precipitation, then DEAE- celluloseion exchange chromatography and Sephadex G-200 for gel filtration chromatography. Specific activity of the purified enzyme by the final purification step was reached to 600 U/mg with 48.8 folds of purification and 45% total recovery.Enzyme molecular weight of about 48 kDadetermined thought sodium dodecyl sulphate polyacrylamide.DPPH and ABTS assays in vitro antioxidanttogether withantitumor effects of L-glutaminase against MCF-7 cell lines by MTT assay indicated an important role of this enzyme in breast cancer.
Keyword: (L-glutamianase, Breast cancer, Antitumor activity, Cytotoxicity )
DOI: https://doi.org/10.31838/ijpr/2020.12.01.053
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