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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Evaluation of anticancer potential for L-glutaminase purified from Bacillus subtilis

Author: ASMAA HUSSEIN, SHYMAA I.ALBARAZANCHI , AHMED F.AL-SHANON
Abstract: In past decade cancer therapy researchgavea promissing forward and many facts suggest thepotenteffects of enzymes depleting amino acids in decreasing the risk of various cancers. In this study L-glutaminase as one of the medically important enzymes purified from Bacillus subtilisusing80% ammonium sulfate precipitation, then DEAE- celluloseion exchange chromatography and Sephadex G-200 for gel filtration chromatography. Specific activity of the purified enzyme by the final purification step was reached to 600 U/mg with 48.8 folds of purification and 45% total recovery.Enzyme molecular weight of about 48 kDadetermined thought sodium dodecyl sulphate polyacrylamide.DPPH and ABTS assays in vitro antioxidanttogether withantitumor effects of L-glutaminase against MCF-7 cell lines by MTT assay indicated an important role of this enzyme in breast cancer.
Keyword: (L-glutamianase, Breast cancer, Antitumor activity, Cytotoxicity )
DOI: https://doi.org/10.31838/ijpr/2020.12.01.053
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