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Activity of I gA Protease secretion by Proteus vulgaris From Urinary Tract Infection Patients
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| Author:
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HANAN SUBHI, FATIMA RAMMADAN ABDUL
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| Abstract:
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A proteolytic strains of Proteus vulgaris were isolated from 18 urinary tract infection
(UTI
)patients of both sexes
,10 were female
(38.5%
) and 16 from male
(61.5 %
),all strainsproduced IgA protease. The highly protease
production was obtained by Proteusvulgaris number
(16
) of 27 mm in diameter after incubation for 18-24 hrs. at
o
37
C on skin milk agar medium and 0.16 mg/ml quantitatively by Bradford method. Isolation and purification of IgA
protease from isolate number
(16
) on Sephadex G100 column which showed the presence of two peak of proteins
and presence of one peak of enzyme.The molecular weight of the partially purified enzymeby gel filtration method in
the 50KDa, there is a correlation between the clinical source strains and the protease molecular weight 50KDa. The
purification fold by SDS-PAGE was
(219.04
) and
(6.1
)yield.The effect of the enzyme on the immunoglobulines was
studied and the results showed the enzyme have the abilities to digest IgA as detected by SDS-PAGE method and the
IgG digestive was not clear. This study determinep.vulgarisinfectionactivity by measurement levels of immunoglobulin
A in 18 patients and 10 healthy as a control groups. The results showed significant decrease
(P<0.05
) in
immunoglobulin A of p. vulgaris infection patients in compared to healthy group.
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| Keyword:
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Ig A, protease, Immunoglobulin,proteus vulgaris, Urinary Tract Infection
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.01.049
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| Download:
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