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An advancement of collagen and curcumin nanoparticles with surfactan using sld method for wound healing application: experimental study on rabbits
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| Author:
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SEPTIANA INDRATMOKO, ANDI TENRI NURWAHIDAH , MAULIDINA INTEN AMRI, ELISA ISSUSILANINGTYAS
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| Abstract:
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Wounds are the loss or damage of some body tissues. Collagen is a key component in the process of wound
healing in the formation of tissues and organs. In addition to collagen, curcumin can also accelerate wound
healing. To improve its potential application, the formulation is carried out by making nanoparticles.
Determination of the optimum formula is using the Simplex Lattice Design method. This study aimed to
determine the effect of collagen and curcumin nanoparticle spray with tween 80 and PEG 400 as rabbits
wound healers (Orytolagus cuniculus). This research was carried out experimentally. The test animals used
were 5 male rabbits (Oryctolagus cuniculus), divided randomly into 5 groups. Consists of negative control,
positive controls (povidone iodine), and collagen nanoparticles spray dose 1, dose 2, and dose 3 groups.
Observations were made by measuring the length of the wound and the time of wound closure. Data were
analyzed by One Way Anova and Post Hoct LSD to determine differences between treatment groups. The
optimum result of tween 80, PEG 400 and shark results in a ratio of 6: 1: 1. The results of particle size were
17.4 nm, zeta potential profile was -2.0 mV, maximal drug loading 125 mg / 5mL and nanoparticle stability for 2
weeks. The results of the best wound healing activity test in rabbits were dose 3 with the concentration of
collagen nanoparticle spray (0,145 mg) and curcumin (0,08 mg) with wound closure time on 7th day. Collagen
and curcumin spray nanoparticles have a wound healing effect that was faster than positive control and
negative control, but did not produce significant differences between groups.
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| Keyword:
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Curcumin, Collagen, Wounds, Nanoparticles, Simplex Lattice Design.
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| EOI:
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| DOI:
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