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Design and Development of Indian Propolis Loaded Poly (e -Caprolactone) Nanoparticles For Improved Anticancer Efficacy
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| Author:
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DR. SATHIYANARAYANAN LOHIDASAN, HARSHAD KAPARE , ARULMOZHI S , KAKASAHEB MAHADIK
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| Abstract:
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Propolis, a natural product obtained from apiculture and is well proven for its anticancer potential. Anticancer efficacy of propolis is limited to its poor water solubility and bioavailability. The present study was designed and investigated for development of ethanolic extract of Indian propolis (EEIP) loaded Poly (e-caprolactone) nanoparticles (EPL) to achieve improved solubility, sustained drug release and enhanced cytotoxic efficacy of EEIP. Formulation development, characterization and optimization were carried out by design of experiment approach. Developed formulations were evaluated in detail for nanoparticle characterization and in vitro cytotoxicity study. Developed nanoparticles showed particle size, and encapsulation efficiency of 190 ± 1 - 230 ± 2 nm and 62.66± 1.20 - 67.18± 1.80 % respectively. Optimized formulation EPL showed sustained drug release over a period of 48 h. Concentration of the drug needed for total growth inhibition of cells in a designed time period (TGI) was decreased by 33.06% for EPL as compared to EEIP in human breast cancer MCF-7 cells and 22.31% in human colon cancer cells HT-29 indicating improved cytotoxicity of EEIP. The study proven that the optimized EPL exhibited increased solubility, sustained drug release and enhanced in vitro cytotoxicity on MCF-7 and HT-29 cell lines in comparison with EEIP. Thus developed system may be served as potential nanocarrier in anticancer treatment.
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| Keyword:
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Indian propolis; Poly (e-caprolactone); Design of experiment; Sulforhodamine B (SRB) assay
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| DOI:
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