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SIMULTANEOUS DETERMINATION OF OFLOXACIN AND PREDNISOLONE ACETATE IN BINARY MIXTURE BY ABSORBANCE CORRECTION METHOD.
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| Author:
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PATEL DHARMENDRAKUMAR, SATISH A. PATEL, BANSARI D. PATEL
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| Abstract:
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A simple and sensitive spectrophotometric method based on absorbance correction was developed for the simultaneous estimation of Ofloxacin and Prednisolone Acetate in binary mixture. Absorbance correction method involves measurement of absorbance at 308 nm for estimation of Ofloxacin and measurement of corrected absorbance at 243 nm for estimation of Prednisolone acetate. The two drugs comply with beer’s-lambert’s law over the linearity range of 2-16 µg/ml for Ofloxacin and 5-35 µg/ml for Prednisolone acetate. The method was validated as per ICH guidelines in terms of linearity, accuracy (recovery study), precision (repeatability, intraday, interday precision), limit of detection and limit of quantification. All the validation parameters were found to be within acceptable limits. The method was found to be simple, sensitive, rapid, cost effective, accurate, and precise for the routine analysis of both the drugs in binary mixture.
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| Keyword:
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Ofloxacin, Prednisolone Acetate, Absorbance correction, Binary mixture, Validation.
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| EOI:
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| DOI:
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