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Assessment of anti-quorum sensing potential of selected medicinal plant extracts using Chromobacterium violaceum
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| Author:
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GUNJANKUMAR MEHTA, VASANTBA J. JADEJA
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| Abstract:
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Quorum sensing is recognized as potential target for developing new antimicrobial agents. In present study, antiquorum
potential of plant extracts was investigated using wild type bioreporter bacterial strain Chromobacterium
violaceum MCC 2290 (ATCC® 12472™). It produces Violacein (violet colour) pigment in a quorum sensing dependent
manner. Chromobacterium violaceum CV026 MCC 2216, a Violacein negative mutant was used to determine mode of
action of crude extracts. Out of total 25 plants screened, the anti-quorum sensing properties were found in crude
extracts of Punica granatum L., Terminalia bellirica Roxb. and Terminalia chebula Retz. with varying degree of effect.
Most potent anti- quorum sensing activity was found in Punica granatum L. with 41.92 % Violacein inhibiton. All the
extracts were found effective at low concentration = 0.075 mg/ml. The presence of anti-quorum sensing
phytoconstituents and their chemical nature were analysed by thin layer chromatography which indicated them to be
polar. The inhibitory effect of plant extract on Violacein production was assessed by qualitative and quantitative
methods.
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| Keyword:
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Quorum sensing inhibition, Chromobacterium, Phytomedicine, Antibiotic resistance
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.04.038
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| Download:
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