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Potential ActivityofNaringeninagainst Renal Ischemia Reperfusion Injury in Male mice
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| Author:
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WIDAD AL-JABBAR, HUSSEIN ABDULKADHIM, WADDAH MAHBOBA, GHIZAL FATIMA , NAJAH R HADI
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| Abstract:
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Acute renal failure is a result of renal ischemia, the development of chronic and advanced renal disorder
and/or incomplete recovery of renal function are the consequent of acute renal failure,most surviving people
are thought to return complete renal function despite a high mortality approximately 50%, the regeneration of
renal tissue and function is attributed to the ability of the kidney to repair cellular damage aimingto investigate
the potential activity of Naringenin in amelioration of renal ischemia reperfusion in male mice.Forty male Swiss
Albino mice weighting 30-35 gram, 10-15 weeks were divided into four groups, ten mice in each group, animal
groups are coordinated as follows: Group I (Sham group): the animals were subjected to the same anesthetic
and surgical laparotomy but without induction of Ischemia Reperfusion Injury (IRI),Group II (Control group):
exposed to bilateral renal IR procedure under anesthesia, Group III (Vehicle group): received vehicles sterile
dimethyl sulfoxide (DMSO 0.2 %) alone intraperitoneal 30 minutes beforeexposed to renal IR procedures
under anesthesia, Group IV (Naringenin treated group ): receivingNaringenin in a dose of 20 mg/kg
intraperitoneal injection 30 minutes before exposed to IR procedure under anesthesia. At the end of
reperfusion period, mice were sacrificed; blood samples were collected directly from thecarotid vein for
determination of serum levels of urea andcreatinine. Bilateral nephrectomy was done and homogenized for
measurements of tissue markers (TLR-2, NF-KB, MCP-1, HMGB-1 and F2 Isoprostane)and before
homogenization part of the kidneys was fixed in formalin for histological examination. The Naringenin
attenuates renal ischemia reperfusion injury through their pleiotropic effects via modulation of the
inflammatory pathway and oxidativeactivity.
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| Keyword:
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Renal Ischemia, Reperfusion Injury,Naringenin, F2-Isoprostane
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.04.079
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| Download:
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