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Production of a therapeutically important Lasparginase enzyme under optimum conditions from Bacillus subtilusmutant isolate
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| Author:
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ASMAA HUSSEIN, MAYS T. ABDALLAH, HADEEL M. KHALAF
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| Abstract:
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L-asparaginase from microbial sources achieved big attention as a therapeutic agent for the treatment of some human
cancers. In this work extra-cellular L-asparaginase produced from Bacillus subtilis mutant using submerged
fermentation process. Bacillus subtilis was tested for L-asparaginase production using M9 medium. The isolate has
been irradiated by physical method (UV irradiation by exposing the wild type to different doses of irradiation (1, 2, 3,
4, and 5 Jole/m) and irradiated eight mutants out of 60 (13.3 %) were found be able to produce enzyme with specific
activity in crude filtrate for the most efficient over producer mutant (B1U1) was 1.5 U/mg protein in comparison to
0.95 U/mg protein for the wild type. Maximum production of L-asparginase was obtained by this mutant after
supplementation of the minimal salt medium (pH7.5) with 0.1% glucose, 0.1% tryptone, incubated at 37oC for 24h.in
shaker incubator. Under these conditions, L-asparginase production in culture supernatant was reached6 U/mg
protein.
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| Keyword:
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L-asparginase, Bacillus subtilis, UV irradiation, Optimum conditions
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.04.005
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| Download:
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