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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Total Phenol Content and Antioxidant activity of Various Concentration of Ethanol Extract of Psidium Guajava L

Author: MUSTOFA AHDA, WAHYU TIKA LESTARI, RIRIN LESTARI SRI RAHAYU
Abstract: Objective: This research has studied the total phenolic content and antioxidant activity from extract ethanol of Psidium Guajava L and also determined both the total phenolic content and antioxidant activity correlation. Methods: The extraction processes of guava leaf used ethanol solvents with different concentrations that were ethanol 96%, 80%, 50% 20% and also water. The guava leaf extract obtained will be identified the phytochemical component and determined the total phenol content using Folin Ciocalteu method and also verified their antioxidant activity to DPPH. Results: Generally, the extracts of Psidium Guajava L. contain some active compounds as phenol content, saponin, flavonoid, and alkaloid. However, the effect of solvent in the extraction lead the different active compound from Psidium Guajava L. The water extract of Psidium Guajava L does not contain any active compound like ethanol extract of Psidium Guajava L. Whereas, the water extract of Psidium Guajava L has less amount total phenolic content and less potency of antioxidant activity than the ethanol extract of Psidium Guajava, possessing as good antioxidant. Conclusion: This research has indicated that the different solvents can lead and result in the different species of the active compounds. While the 80% ethanol extract of Psidium Guajava L produced the highest antioxidant activity with the IC50 value of 7.73±0.7 µg/mL.
Keyword: Total Phenol Content, Antioxidant Activity, Psidium Guajava L
DOI: https://doi.org/10.31838/ijpr/2019.11.03.078
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