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Total Phenol Content and Antioxidant activity of Various Concentration of Ethanol Extract of Psidium Guajava L
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| Author:
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MUSTOFA AHDA, WAHYU TIKA LESTARI, RIRIN LESTARI SRI RAHAYU
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| Abstract:
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Objective: This research has studied the total phenolic content and antioxidant activity from extract
ethanol of Psidium Guajava L and also determined both the total phenolic content and antioxidant
activity correlation.
Methods: The extraction processes of guava leaf used ethanol solvents with different concentrations
that were ethanol 96%, 80%, 50% 20% and also water. The guava leaf extract obtained will be identified
the phytochemical component and determined the total phenol content using Folin Ciocalteu method and
also verified their antioxidant activity to DPPH.
Results: Generally, the extracts of Psidium Guajava L. contain some active compounds as phenol
content, saponin, flavonoid, and alkaloid. However, the effect of solvent in the extraction lead the
different active compound from Psidium Guajava L. The water extract of Psidium Guajava L does not
contain any active compound like ethanol extract of Psidium Guajava L. Whereas, the water extract of
Psidium Guajava L has less amount total phenolic content and less potency of antioxidant activity than
the ethanol extract of Psidium Guajava, possessing as good antioxidant.
Conclusion: This research has indicated that the different solvents can lead and result in the different
species of the active compounds. While the 80% ethanol extract of Psidium Guajava L produced the
highest antioxidant activity with the IC50 value of 7.73±0.7 µg/mL.
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| Keyword:
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Total Phenol Content, Antioxidant Activity, Psidium Guajava L
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.03.078
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| Download:
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