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Multi-locus Sequence Typing of Multi-Drug Resistant Escherichia coli isolated from Urinary Tract Infections
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| Author:
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AYA, KEMAAL, DHAFAR, N. AL-UGALI, ISSAM, S. AL-AZZAWI
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| Abstract:
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Urinary tract infection is the most common community and hospital acquired bacterial infection in persons of human
characterized by a high rate of treatment failure and recurrence of infection. UropathogenicE. coli(UPEC)was the main
pathogen isolated from urine of patients suffering from UTIs. Increasing resistance to several commonly prescribed
antibiotics by uropathogenicE. coli is a major concern worldwide. In this study, three hundredurine samples were
collected from hospitals in Baghdad. One hundred twenty E coli isolates were identified. Antibiotic sensitivity test was
used for the detection of the susceptibility of the E coli isolates against eleven different antibiotics. Results revealed a
high sensitivity levelof these isolates to Imipenem (97.5%) followed by Amikacin (91.6%), Nitrofurantoin (88.33%),
Gentamicin (71.6%), Levofloxacin (55%) and Doxycycline (45%). On the other hand, a high level of resistance to
Amoxicillin-clavulanic acid (90%) compared with moderate level of resistance to Ceftriaxone (52.5%), Cefotaxime
(46.6%), Trimethoprim (43.3%) and Ciprofloxacin (30%). Multilocus sequence typing (MLST) was used to identify
sequencing of the types of UPEC by using eight housekeeping genes(dinB, icdA, pabB, polB, putP, trpA, trpB, uidA.As it is
compared with MLST database, the sequence type revealed that there was no completely matched for all stains with
MLST database while the phylogenetic tree showed a link between the ten selected isolates. There is a genetic
relatedness between the isolates that obtained from the same source and showed the same resistance towards used
antibiotics. MLST has a high discriminatory power in sequencing and typing of UPEC and the allelic profiles of isolates
can easily be compared to those in a large central database via the Internet.
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| Keyword:
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UropathogenicE. coli (UPEC), Urinary tract infection UTI, Multilocus sequence typing (MLST),Antimicrobial resistance (AR).
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.03.042
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| Download:
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