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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Multi-locus Sequence Typing of Multi-Drug Resistant Escherichia coli isolated from Urinary Tract Infections

Author: AYA, KEMAAL, DHAFAR, N. AL-UGALI, ISSAM, S. AL-AZZAWI
Abstract: Urinary tract infection is the most common community and hospital acquired bacterial infection in persons of human characterized by a high rate of treatment failure and recurrence of infection. UropathogenicE. coli(UPEC)was the main pathogen isolated from urine of patients suffering from UTIs. Increasing resistance to several commonly prescribed antibiotics by uropathogenicE. coli is a major concern worldwide. In this study, three hundredurine samples were collected from hospitals in Baghdad. One hundred twenty E coli isolates were identified. Antibiotic sensitivity test was used for the detection of the susceptibility of the E coli isolates against eleven different antibiotics. Results revealed a high sensitivity levelof these isolates to Imipenem (97.5%) followed by Amikacin (91.6%), Nitrofurantoin (88.33%), Gentamicin (71.6%), Levofloxacin (55%) and Doxycycline (45%). On the other hand, a high level of resistance to Amoxicillin-clavulanic acid (90%) compared with moderate level of resistance to Ceftriaxone (52.5%), Cefotaxime (46.6%), Trimethoprim (43.3%) and Ciprofloxacin (30%). Multilocus sequence typing (MLST) was used to identify sequencing of the types of UPEC by using eight housekeeping genes(dinB, icdA, pabB, polB, putP, trpA, trpB, uidA.As it is compared with MLST database, the sequence type revealed that there was no completely matched for all stains with MLST database while the phylogenetic tree showed a link between the ten selected isolates. There is a genetic relatedness between the isolates that obtained from the same source and showed the same resistance towards used antibiotics. MLST has a high discriminatory power in sequencing and typing of UPEC and the allelic profiles of isolates can easily be compared to those in a large central database via the Internet.
Keyword: UropathogenicE. coli (UPEC), Urinary tract infection UTI, Multilocus sequence typing (MLST),Antimicrobial resistance (AR).
DOI: https://doi.org/10.31838/ijpr/2019.11.03.042
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0.12
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