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Evaluating the role of Homeopathic medicine Testosterone in its various attenuations for its anticancer activity on Breast Cancer cell line MCF-7.
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| Author:
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DALJEET KAUR, JAY NILESH SHAH, RAHUL M. SONAVALE
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| Abstract:
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The second most prevalent type of cancer which is also the leading cause of women death in India is Breast Cancer. In
its treatment several recommendation and measures are considered. Androgen receptors have been found on breast
cancer cell lines and testosterone has been used as hormone therapy against breast cancer. Homeopathic medicines
are part of complementary and alternative medicines are used for the treatment of cancer. The objective of the study
is to investigate and evaluate the anticancer activity of commercially available homeopathic preparations of
Testosterone. To test the viability of breast cancer (MCF-7) cell line, 3-(4,5-Dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide (MTT) assay was conducted with negative control, ethanol and various attenuations of
homeopathic medicine Testosterone at 24 hours. Ethanol was tested to be safe to the cells up to 3µl thereby
coherent to the cells being viable up to 3% of alcohol. Multiple comparisons between tested reagents at different
concentrations confirmed the significance of the said results. At concentration of 1:33, 6CH and 200CH potency
shows superior activity in comparison to negative control, ethanol, 30CH and 1M potency (p<0.001). These
preliminary significant results warrant further in vitro and in vivo studies to evaluate the potential of Testosterone as a
medicine to treat breast cancer.
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| Keyword:
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Homeopathy, Breast cancer, Testosterone, in vitro, Anticancer activity.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.03.025
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| Download:
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