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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Evaluating the role of Homeopathic medicine Testosterone in its various attenuations for its anticancer activity on Breast Cancer cell line MCF-7.

Author: DALJEET KAUR, JAY NILESH SHAH, RAHUL M. SONAVALE
Abstract: The second most prevalent type of cancer which is also the leading cause of women death in India is Breast Cancer. In its treatment several recommendation and measures are considered. Androgen receptors have been found on breast cancer cell lines and testosterone has been used as hormone therapy against breast cancer. Homeopathic medicines are part of complementary and alternative medicines are used for the treatment of cancer. The objective of the study is to investigate and evaluate the anticancer activity of commercially available homeopathic preparations of Testosterone. To test the viability of breast cancer (MCF-7) cell line, 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay was conducted with negative control, ethanol and various attenuations of homeopathic medicine Testosterone at 24 hours. Ethanol was tested to be safe to the cells up to 3µl thereby coherent to the cells being viable up to 3% of alcohol. Multiple comparisons between tested reagents at different concentrations confirmed the significance of the said results. At concentration of 1:33, 6CH and 200CH potency shows superior activity in comparison to negative control, ethanol, 30CH and 1M potency (p<0.001). These preliminary significant results warrant further in vitro and in vivo studies to evaluate the potential of Testosterone as a medicine to treat breast cancer.
Keyword: Homeopathy, Breast cancer, Testosterone, in vitro, Anticancer activity.
DOI: https://doi.org/10.31838/ijpr/2019.11.03.025
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