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Possible Roles of miRNAs-25 and 375 as Potent Biomarkers for ß-cellsStress and/or Death in DiabetesType 1 Patients and their First Degree Relatives
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| Author:
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, MADHA MOHAMMED SHEET, HASAN ABD ALI KHUDHAIR
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| Abstract:
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Diabetes Type 1 (T1D) is an autoimmune condition represent by pancreatic ß-cells loss due to autoimmune processes,
which reflected by presence of autoantibodies directed against islets ß-cells auto-antigens. This study was aimed to
identify the predictive power and the degree of correlation of genetic biomarkers (miRNA-25 and miRNA-375) on ß-
cells stress and/or death in T1D individuals and their first degree relatives (FDRs). Three subjects of T1D individuals,
FDRs and apparently healthy individuals were enrolled in this study and evaluated for their serum C-peptide and
expression level of miRNA-25 and miRNA-375. The results showed that the frequency of normal level and mean titer
of C-peptide were significantly low among T1D and their FDRs. The frequency of high level and mean titer of miRNA-
25 were significantly low in both groups. For miRNA-375, the results revealed low level in 35% of T1D patient and in
10% of FDRs, whereas the mean titer was significantly higher among FDRs in comparison to HC. The miRNA-25 level
was significantly lower in subjects with below normal C-peptide than subjects with normal or above normal C-peptide.
Furthermore, miRNA-25 level was statistically highest in individuals with low miRNA-375 level than those with high
level. Finally, a significant elevationinmiRNA-375 level was noticed among the individuals with below normal C-peptide
compared to individuals with normal or above normal level of C-peptide. In conclusion, miRNA-25 had expressed a
decreased level among the new onset T1D patients and their FDR, whereas miRNA-375 had expressed decreased level
among newly onset T1D and elevated level among FDRs. The correlation between the residual ß-cells mass and the
miRNA-25 serum level was positive which indicate the possible utility of its low expression as a ß-cells stress and/or
death predictive biomarker. On the other hand the correlation between the residual ß-cells mass and the miRNA-375
serum level was negative which indicate the possible utility of its high expression as a ß-cellsstress and/or death predictive
biomarker.
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| Keyword:
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Diabetes Type 1, micro-RNAs and ß-cells stress/death
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.03.023
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| Download:
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