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Protein Map Standardization of Human Urine Using Two Dimensional Gel Electrophoresis (2-DE)
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| Author:
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, SAEID REZA DOUSTJALALI, NEGAR SHAFIEI SABET, AIDA NURASHIKIN ABD RAHMAN, NYAN HTAIN LINN, KARIM AL-JASHAMY, AHMAD TAHA KHALAF, KHIN THANT ZIN, S. SARAVANA KUMAR, HLAING THAW DAR, KHIN TO, SAMIAH YASMIN ABDUL KADIR, JAMALUDIN ZAINOL, ROHAINI MOHAMAD, AB HALIM MANSAR, REBECCA SY WONG, NORALIZA A, NOR DATIAKMA MA, MARZALINA MANSOR, MOHD NASIR MOHD DESA AND WON FEN WONG
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| Abstract:
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The changes of urinary proteome reflect disease-related changes of tissue or cellular proteomes and gives urine
enormous potential for early detection of disease, classification of disease, choice of therapeutic agents, assessment of
prognosis, and monitoring of a particular therapeutic regimen. Urine is available in almost all patients at relatively large
quantities and the collection of urine is simple and noninvasive as compared to other bio-fluids, urine can be
considered as one of the most valuable bio-fluids for biomarker discovery. In this study, the human urine was used
and we established a reproducible and optimized protocol to display the two dimensional gel electrophoresis (2-DE)
protein map of human urine. The standard optimization of the 2-DE protein mapping at pH 3-10was achieved with 60
µg proteins loading. This protocol further could be used along with identification of differentially expressed proteins
by mass spectrometry when 2-DE protein map of patients urine are compared to normal healthy individuals. These
differentially expressed proteins could be later used as specific and sensitive biomarkers for early diagnosis and
prognosis of the disease in question. The procedure used in our study generated a highly reproducible and optimized
reference 2-DE protein mapping of human urine.
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| Keyword:
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Proteomics, 2-DE, human urine
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.03.012
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| Download:
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