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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

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0975-2366
5 - Years Impact Factor

Year 2012 - 2016

Impact Factor: 1.55

Total Publications: 317

Total Citation: 491

Year 2011 - 2015

Impact Factor: 1.46

Total Publications: 326

Total Citation: 477

Year 2010 - 2014

Impact Factor: 1.3

Total Publications: 313

Total Citation: 407

Year 2009 - 2013

Impact Factor: 0.973

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Total Citation: 285

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ESTIMATION OF TOCOPHEROL (VIT-E) CONTENT IN DIFFERENT EDIBLE OILS BEFORE AND AFTER HEATING

Author: K.SAI KRISHNA, P.ABHINANDANA, PROF.N.RAMARAO, M. RAVIKUMAR, PROF.M.S.UMASHANKAR
Abstract: The endeavor of the present work was to develop and validate a simple, efficient, economical and novel green analytical method for the estimation of a-tocopherol in oil samples by three different methods. Method A involves measurement of absorbance at 2975 cm-1 by FT-IR spectrophotometer. Method B was First derivative method at 285nm by UV-spectrophotometer. Method C was Calibration method at 285nm by UV-spectrophotometer. Three methods were validated according to ICH guidelines. Linearity was found in the range of 1-9µg/ml subject to Infrared spectroscopy and 10-90µg/ml subject to UV spectroscopy. The %RSD was found to be less than 2 and %recovery is within 95% -105% for all three methods. We analyzed the tocopherol content in four oils samples before heating and content after heating at 700C and 1000C. Then we experiential the effect of heating on a-Tocopherol content of different vegetable oils. Thermal degradation of a-Tocopherol in oil samples was minimal at 700C and increased to 20% at 1000C. From the domino effect we can make out that improper heating of oil’s leads to degradation of a- Tocopherol.
Keyword: Tocopherol, FT-IR spectrophotometer, First derivative.
DOI: https://doi.org/10.31838/ijpr/2019.11.03.007
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Impact Factor for five years is 1.55 (2012 - 2016)

Year 2011 - 2015 Impact Factor - 1.46 Total Publications - 326 Total Citations - 477