|
Development and Validation of First Order Derivative Spectrophotometric Method for Simultaneous Estimation of Rutin Trihydrate and Trigonelline Hydrochloride in Antidiabetic Herbal Formulation
|
|
| Author:
|
PALAK CHAUDHARY, DR. HARSHA U. PATEL
|
| Abstract:
|
A simple, fast, accurate, reliable and economical first order derivative spectrophotometric method was developed for
the simultaneous determination of Rutin trihydrate (RUT) and Trigonelline hydrochloride (TRG) in herbal
formulation. Rutin and Trigonelline are present as markers in antidiabetic herbal formulations to prevent and manage
diabetes. The Zero crossing point (ZCP) of markers was determined at which no interference from the other
marker. The developed method gives best results in terms of linearity, accuracy, precision, limit of detection (LOD)
and limit of quantification (LOQ). The method was validated as per ICH guidelines. The linearity range for Rutin
trihydrate and Trigonelline hydrochloride were found to be 5 to 25 µg/ mL and 5 to 25 µg/mL respectively. The
values of LOD were 0.50 µg/ mL and 0.18 µg/mL and the values of LOQ were found to be 1.50 µg /mL and 0.55 µg/
mL for Rutin trihydrate and Trigonelline hydrochloride respectively.
|
| Keyword:
|
Rutin trihydrate (RUT), Trigonelline hydrochloride (TRG), First order derivative spectroscopy, Validation
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2019.11.02.092
|
| Download:
|
Request For Article
|
|
|