*Five Years Citation in Google scholar (2016 - 2020) is. 1451*   *    IJPR IS INDEXED IN ELSEVIER EMBASE & EBSCO *       

logo

INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence
Published by : Advanced Scientific Research
ISSN
0975-2366
Current Issue
Article In Press
No Data found.
ADOBE READER

(Require Adobe Acrobat Reader to open, If you don't have Adobe Acrobat Reader)

Index Page 1
Click here to Download
IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

Click to download
 

Article Detail

Label
Label
Development and validation of HPTLC method for estimation of Tribulosin; an important biomarker in Indian Tribulus terrestris

Author: PRAJAKTA KULKARNI, KAKASAHEBMAHADIK, SATHIYANARAYANANLOHIDASAN
Abstract: A simple, accurate and precise HPTLC (High Performance Thin layer Chromatography) method was developed for quantification of Tribulosin in Tribulus Terrestris L which is well known for the treatment of infertility. Tribulosin is one of the markers which are not sensitive to UV (ultra violet) detection hence for quantification of such marker different analytical techniques can be used. Determination of Tribulosin was carried out using pre-coated silica gel 60 F254 TLC platesas stationary phase. Ascending development was followed in twin trough chamber using n-propanol: toluene: water: glacial acetic acid (6:2.5:1:0.5 v/v). The developed method gave yellow coloured spots of Tribulosin (Rf 0.46). A good linear relationship was obtained in the range of 200-1200ng. The correlation coefficient of 0.997 concluded good dependence of peak area on concentration. The amount of Tribulosin in butanol fraction of Tribulus Terrestris Lwas found to be 0.193mg/100mg w/w. The method was validated in terms of Linearity, LOD, LOQ, Robustness Accuracy, Precision and Specificity as per ICH (International Conference on Harmonisation) guidelines. Recovery study showed values between 96-98% which determines the accuracy of the method. The limit of detection and quantification were found to be 72.50 and 219.70 ng/spot respectively. The developed method can be used to standardise extracts or herbal formulations of Tribulus Terrestris L for Tribulosin.
Keyword: Tribulus Terrestris L, Tribulosin, HPTLC, validation
DOI: https://doi.org/10.31838/ijpr/2019.11.02.090
Download: Request For Article
 




ONLINE SUBMISSION
USER LOGIN


Username
Password
Login | Register
News & Events
SCImago Journal & Country Rank

Terms and Conditions
Disclaimer
Refund Policy
Instrucations for Subscribers
Privacy Policy

Copyrights Form

0.12
2018CiteScore
 
8th percentile
Powered by  Scopus
Google Scholar

hit counters free