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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Differential Expression of Antioxidant Enzymes in selective Human Pathogenic Organisms under the Influence of Indian Spices

Author: HEMA A, NEETIN DESAI, R. SWAMINATHAN, K. JAIN
Abstract: In last few years there has been dramatically increase in search of alternative for antibiotics aimed at a partial or total replacement of chemical antibiotics. Indian Spices and their extracts possess antimicrobial activity through several different pathways, including direct inhibition of reactive oxygen species, iron chelation and antioxidant genes induction. In present study, inhibitory concentration of ethanolic extract of Cinnamomum zeylanicum (Dalchini), Syzygium aromaticus (Clove) and Trachysparmum ammi L. (Ova) was optimized against five human pathogens viz; Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, and Salmonella typhi. We have expanded our investigation by determining the activities of Catalase (CAT), Superoxide Dismutase (SOD), Mnperoxidase (Mn-P) and Laccase in human pathogens in presence and absence of spice extracts. The results indicated that 15µg/ml, 20µg/ml and 25 µg/ml concentration of spice extracts influenced the growth of organisms as compared to untreated organisms. Results of antioxidant enzyme activities revealed that intracellular activity of CAT, SOD, Mn- P and Laccase was comparatively high in untreated cells of all organisms. Further it was observed that under the influence of dalchini extract (5µg/ml, 15µg/ml, 25µg/ml) Mn-P and Laccase activities in all organisms increased as compared to untreated cells. Expression level of all antioxidant enzymes in supernatant as well as in cell lysate was significantly different in treated and untreated cells (P< 0.01). The change in enzyme activities indicates the reduction of reactive oxygen species in the pathogenic organisms which might protect the organism from mutations. Thus, combination of spice extracts with antibiotics may help to reduce antimicrobial resistance in human pathogens.
Keyword: Catalase (CAT), Superoxide Dismutase (SOD), Mn-peroxidase (Mn-P), Laccase Human pathogens, Resistance, Spices.
DOI: https://doi.org/10.31838/ijpr/2019.11.02.068
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