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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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ECO-FRIENDLY SYNTHESIS OF SILVER NANOPARTICLES USING AQUEOUS LEAVES EXTRACT OF CLOME GYNANDRA AND THEIR ANTIBACTERIAL ACTIVITY”

Author: ASHA SIVAJI, A. ASHA, S. RAJESHKUMAR
Abstract: In the current work green chemistry mediated silver nanoparticle was synthesized usingan aqueous leaf extract of clome gynandra. The silver nanoparticles formation was primarily confirmed by the colour change of plant extract and silver nitrate solution. The aqueous leaf extract and aqueous silver nitrate solution on reduction changed the colour from yellowish to reddish brown. Synthesis of AgNPs was further confirmed from theUV-VIS spectroscopy study. Fourier transform infra-red (FT-IR) analysis of leaf extract and AgNPs indicated the presence of various functional groups as phytochemical constituent. The antibacterial activity of aqueous extract and silver nanoparticles was confirmed by the well diffusion method. The antimicrobial property of silver nanoparticles against Escherichia coli, Salmonella sp., Bacillus sp., Proteus sp. and Klebsiella pneumonia was analysed by measuring the inhibition zone. The bacterial growth was inhibited in the following order Escherichia coli, Klebsiella pneumonia, Salmonella sp., Proteus sp. and Bacillus sp.,by the synthesized silver nanoparticle at both concentrations of 10 µl and 20 µl, while aqueous leaf extract ofCleome gynandra does not exhibit any antibacterial activity at 10 µl concentration. The positive control also exhibited a maximum antibacterial activity at 10 µl concentration. Thus it can be concluded that the silver nanoparticles may have asignificant improvement over conventional antibiotic drug.
Keyword: Green synthesis, Cleome gynandra, silver nitrate, silver nanoparticle, antibacterial activity.
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