|
Biocompatible novel carbohydrate polymer mannan sulphate: Biophysicochemical characterization and cytotoxicity evaluation
|
|
| Author:
|
VARSHA POKHARKAR, MEGHA V. MUGADE
|
| Abstract:
|
The carbohydrate polymers used in nanoparticles synthesis for targeted delivery is mainly preferred based upon their specificity and biocompatibility. The mannose receptor expression plays a pivotal role through strongest binding of sulphated polysaccharides with cysteine-rich (Cys-Rich) domain. In an effort to search biocompatible novel carbohydrate polymer for targeted drug delivery this study aims to synthesis and evaluation of sulphated polysaccharide mannan sulphate (MS). Mannan sulphate was synthesized from mannan isolated commercially from the cell wall of baker’s yeast (Saccharomyces cerevisiae).The mannan sulphate was characterized for percentage yield (86.66%), molecular weight determination (Mw=5.3 X 104 g/mol), degree of substitution (DS=1.23), total sugar and sulphate content (74.5±0.3% & 45.5± 1.3%) by using Ostwald method, elemental analyzer technique, phenol sulphuric acid method and Kawai method respectively. The protein content was estimated by Bradford method. Further, UV, FTIR and 13 C NMR were used for characterization of MS. The cytotoxicity studies were performed in cell lines such as murine macrophages J774A.1 cells, human skin fibroblasts TE 353.Sk cells and human keratinocytes HaCaT cells which exhibited a significant biocompatibility of mannan sulphate. This synthesis and cytotoxicity evaluation together could contribute to the development of biocompatible nanoparticles synthesis, which may aid in targeting drugs to specific parts of human body containing cells which expresses mannose receptor. This study concludes that mannan sulphate with interesting physicobiochemical properties may serve as valuable lead for nanoparticles synthesis for the treatment of human diseases.
|
| Keyword:
|
Mannan, Mannan sulphate, Carbohydrate polymer, MTT assay, biocompatible
|
| EOI:
|
-
|
| DOI:
|
-
|
| Download:
|
Request For Article
|
|
|